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Fig. 13 | Particle and Fibre Toxicology

Fig. 13

From: Respiratory and systemic impacts following MWCNT inhalation in B6C3F1/N mice

Fig. 13

Changes in macrophage subpopulations in the spleen following 30-day MWCNT exposure + 10 days recovery to MWCNT. At multiple doses of particle exposure, splenic cell populations were assessed for changes using flow cytometry. Spleens were removed and disrupted to achieve single-cell suspensions for analysis. a General particle uptake (presumably by Mφ) measured by side-scatter showed no change across the dose-range compared to Air control. b This graph shows the percent Μφ (CD11b+ MHCIImid/hi Ly6Clo/hi) in the spleen, which decreases significantly at the highest dose ** P < 0.01 compared to all other groups. c This panel shows changes in the inflammatory macrophage subpopulation with significant increases in iMφ (CD11b+ Ly6Chi) for the highest 2 doses compared the low dose †† P < 0.01 and the Air control * P < 0.05. d Shows changes in the resident macrophage subpopulation with significant decreases in rMφ (CD11b+ Ly6Clo) for the two highest doses compared to the lowest dose and the air control ** P < 0.01. Mφ = total macrophages; iMφ = inflammatory macrophages; rMφ = resident macrophages. All data expressed as mean ± SEM (n = 8)

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