Fig. 9

MCC950 induces a concentration-dependent decrease in stone particle-induced IL-1β release in THP-1 macrophages. THP-1 macrophages were exposed to 0–0.1 μM MCC950 and incubated for 30 min before adding 200 μg/mL quartzite (a), anorthosite (b), rhomb porphyry (c), dacite (d), quartz diorite (e) and hornfels (f), and incubating the cells for 24 h. The levels of IL-1β in the cell culture supernatant was detected by ELISA. Results are presented as mean ± SD (n = 4) and are normalized to the mean response of each experiment. Statistical significance is based on a two-way ANOVA followed by Dunnet’s and Sidak post-tests. Values in b were log-transformed prior to statistical analysis to satisfy model assumptions of normality and homoscedasticity. * Statistically significant difference from the respective control. # Statistical significance between exposure groups