Fig. 5
The role of mitochondrial OXPHOS on QFF-PM2.5 caused thrombopoiesis and megakaryocytic maturation. The effects of QFF-PM2.5 exposure on the mRNA levels (a) and protein expressions (b) of mitochondrial respiratory chain complex I-V in megakaryocytes. The antagonistic effects of rotenone on the thrombopoiesis (c) and morphological alterations in megakaryocytes induced by 100 ng/mL QFF-PM2.5 exposure based on bright field microscopic observations with or without Giemsa staining (d, e). f CD33 and CD41a levels in megakaryocytes upon 100 ng/mL QFF-PM2.5 treatment with or without 15 nM rotenone. **p < 0.01. ap < 0.05 versus the negative controls, bp < 0.05 versus the groups with QFF-PM2.5 treatment alone