Fig. 6

Influence of gene interference on A549 cell cycle distribution. For all the interference groups, the cell proportion increased at the G1 phase, while this decreased at the S-phase. After the target gene was interfered, the cell cycle was arrested at the G1 phase. In the siRNA RPL13, siRNA RPL6 and siRNA RPL18A group, the proportion of A549 cells in the G1 phase was 75.08 ± 2.21 %, 68.72 ± 0.63 % and 72.75 ± 1.62 %, respectively, which was higher than that in the blank group (56.19 ± 1.74 %) and NC group (59.73 ± 0.66 %) (P < 0.01). The number of cells in the G1 phase in the siRNA HIS1H4A group was 63.41 ± 0.94 %, but the difference was not statistically significant, when compared to the NC group (P > 0.05). Meanwhile, in the siRNA RPL13, siRNA RPL6, siRNA RPL18A and siRNA HIS1H4A groups, the proportion of A549 cells in the S phase was 10.12 ± 0.40 %, 12.62 ± 2.60 %, 11.27 ± 1.75 %, and 17.31 ± 1.84 %, respectively, which was lower than those in the blank group (28.22 ± 3.01 %) and NC group (26.20 ± 2.98 %) (P < 0.05)