Fig. 6
Evaluation of Iba1 immunostaining in the brain. A Quantification of Iba1 (microglia) positive % area per field at 400x at the end of treatment (EoT) and recovery (Rec) in the frontal cortex and hippocampus. Data are expressed as means ± SD. Statistical significance: * = p < 0.05; ***p < 0.001 vs Ctr (Kruskal-Wallis test, followed by Dunn’s multiple comparison test); a = p < 0.05; b = p < 0.01 vs Ctr (Mann-Whitney test) (n = 5). Ctr = control group. 1 Nominal dose (actual delivered doses were 0.20 and 0.80 mg/kg bw). B Representative images of Iba1 immunostained sections of frontal cortex, scale bar = 50 μm