Skip to main content
Fig. 8 | Particle and Fibre Toxicology

Fig. 8

From: Pulmonary delivery of the broad-spectrum matrix metalloproteinase inhibitor marimastat diminishes multiwalled carbon nanotube-induced circulating bioactivity without reducing pulmonary inflammation

Fig. 8

Evaluation of the role of MWCNT-exposure generated serum bioactivity on brain endothelial barrier integrity. Mouse cerebrovascular endothelial cells (MCEC) grown in a confluent monolayer were treated with serum (5% v/v) from C57BL/6 mice exposed to MWCNT in conjunction with Marimastat (Mar) or Vehicle (Veh). Barrier integrity changes were accessed via Electric Cell-substrate Impedance Sensing (ECIS). a Normalized electric resistance changes across endothelial monolayer were recorded over the course of 24 h. after serum treatment. b Averages of normalized resistance from different groups were compared after 24 h of serum treatment. Serum from MWCNT-treated mice decreased barrier integrity compared to controls. Marimastat partially recovered barrier integrity in MWCNT exposed group although not back to DM/Veh control serum levels. Data presented are means ± SEM. *p < 0.05 compared to DM/Mar. **p < 0.01 compared to DM/Veh. ***p < 0.0001 compared to DM/Veh and DM/Mar. p values based on 2-way ANOVA with Tukey’s multiple comparison test presented for each figure. n = 4 per group

Back to article page

Particle and Fibre Toxicology

ISSN: 1743-8977

Contact us