Fig. 2
From: The mechanism of cell death induced by silver nanoparticles is distinct from silver cations
Sensitivity to AgNPs is independent from sensitivity to silver ion. Representative dose response curves and IC50 following 72 h A AgNP or B Ag+ exposure. Cell viability following 72 h AgNP or Ag+ exposure was quantified by MTT assay and IC50 was determined using GraphPad Prism. Data used to calculate IC50s were obtained from 6 technical replicates per dose and 3 independent experiments (biological replicates). Statistical analysis was performed by two-was ANOVA and post-hoc Tukey Test. Significant differences are indicated (****p < 0.0001). Long-term proliferative potential was assessed via clonogenic assay following 24 h C AgNP or D Ag+ exposure. The data are presented as relative surviving fraction based upon clonogenic growth normalized to the plating efficiency. Statistical analysis was performed by two-way ANOVA followed by post-hoc Tukey Test. Statistical differences are indicated (****p < 0.0001)