Nanomaterial- and shape-dependency of TLR2 and TLR4 mediated signaling following pulmonary exposure to carbonaceous nanomaterials in mice

Table 3 BAL cell counts in mice 24 h post exposure

	Control	LPS	CB	CNT	GO
	0 µg	4 µg	162 µg	54 µg	18 µg
WT
Total BAL cells (× 10³)	89.6 ± 56.3	824 ± 404***	191 ± 61.2**	151 ± 33.6*	293 ± 85.9**
Macrophages (× 10³)	67.2 ± 22.6	76.8 ± 47.3	23.9 ± 15.4***	50.8 ± 8.74	52.4 ± 13.9
Lymphocytes (× 10³)	0.34 ± 0.31	6.14 ± 6.96	1.30 ± 1.35	2.20 ± 1.51*	1.61 ± 1.10*
Neutrophils (× 10³)	12.8 ± 26.9	724 ± 338**	133 ± 45.4***	70.8 ± 31.9*	218 ± 73.9**
Eosinophils (× 10³)	10.2 ± 22.4	1.37 ± 3.06	28.2 ± 23.4*	21.3 ± 15.0	17.1 ± 9.82
Epithelial (× 10³)	5.98 ± 3.86	15.6 ± 14.7	5.52 ± 3.95	5.61 ± 1.94	4.13 ± 2.65
Tlr4 KO
Total BAL cells (× 10³)	89.6 ± 56.3	295 ± 423	178 ± 43.1*	53.2 ± 33.4^b	293 ± 82.0**
Macrophages (× 10³)	67.2 ± 22.6	66.8 ± 28.4	14.8 ± 7.30**	21.2 ± 19.5**^{, a}	50.7 ± 13.8
Lymphocytes (× 10³)	0.34 ± 0.31	0.21 ± 0.42	1.50 ± 0.92*	0.70 ± 0.61	0.82 ± 0.79
Neutrophils (× 10³)	12.8 ± 26.9	219 ± 411	135 ± 31.5**	23.9 ± 11.2*^{, a}	217 ± 78.9**
Eosinophils (× 10³)	10.2 ± 22.4	5.80 ± 8.67	21.4 ± 15.9*	5.03 ± 2.84	19.4 ± 14.8
Epithelial (× 10³)	5.98 ± 3.86	3.50 ± 4.05	4.63 ± 2.47	2.36 ± 1.70	5.48 ± 6.31

All values are presented as mean ± SD. There were no statistical significant difference between the vehicle controls, therefore these were pooled in the statistical analyses (WT + Tlr4 KO). A symbol (*) denotes P ≤ 0.05, (**) P ≤ 0.01, (***) P ≤ 0.001 compared to vehicle control. Comparing Tlr4 KO and WT, a letter (^a) denotes P ≤ 0.05, (^b) P ≤ 0.01

ISSN: 1743-8977