Table 9 Experimental design
| Â | 2017 cohort | 2018 cohort | 2019 cohort |
|---|---|---|---|
Exposure months | March–June | August–November | August–November |
Age at first surgery | 3Â months | 3Â months | 3Â months |
Age at start of exposure | 4Â months | 4Â months | 4Â months |
Exposure duration | 12Â weeks | 12Â weeks | 12Â weeks |
Experimental groups | Gonad intact male Air Gonad intact male PM2.5 Gonad intact female Air Gonad intact female PM2.5 | Sham female Air Sham female PM2.5 OVAX female Air OVAX female PM2.5 | Sham female Air Sham female PM2.5 |
Endpoints | Male and female | Sham and OVAX | Sham |
| Â | BP (tail cuff and transmitter) | BP (tail cuff and transmitter) | Ovarian estradiol Estrous cycling |
| Â | ECG (transmitter) | ECG (transmitter) | Â |
| Â | Â | Oil red O aorta | Â |
| Â | Female only | Sham only | Â |
| Â | Estrous cycling | Ovarian estradiol | Â |
| Â | Follicle counts | Estrous cycling | Â |
|  | Ovarian IHC (PUMA, activated caspase 3, γH2AX, Ki67) |  |  |
| Â | Ovarian TUNEL | Â | Â |