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Fig. 1 | Particle and Fibre Toxicology

Fig. 1

From: Combined exposure to fine particulate matter and high glucose aggravates endothelial damage by increasing inflammation and mitophagy: the involvement of vitamin D

Fig. 1

The effects of PM on the viability and apoptosis of endothelial cells treated with high glucose. HUVECs were pretreated with high glucose (30 mM) for 24 h and then treated with PM (50 μg/mL) for 8 h. A Cell viability was assessed using the MTT assay. B, C Cell death was detected using Annexin V-FITC/propidium iodide (PI) double staining. D Apoptosis was examined and quantified using the TUNEL assay (TUNEL-positive cells: green; nuclei: blue; bar = 50 μm). E, F The levels of cytochrome c and PUMA expression determined using Western blotting. *P < 0.05 compared with the control group; †P < 0.05 compared with the HG group; #P < 0.05 compared with the PM group

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Particle and Fibre Toxicology

ISSN: 1743-8977

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