Fig. 6

Mitophagy and inflammation induced by high glucose and PM in HUVECs were regulated by the JNK and p38 signaling pathways. HUVECs were pretreated with high glucose (30 mM) for 24 h and then treated with PM (50 μg/ml) for 8 h or a specified period. A, B The levels of p-ERK, p-p38, and p-JNK were detected at different time points (0, 0.5, 1, 2, 4, and 8 h) using Western blotting. C HUVECs were pretreated with PD98059 (10 μM), SP600125 (5 μM) or SB203580 (15 μM) for 1 h before PM stimulation. AO was used to detect the formation of autolysosomes. Bar = 50 μm. D, E HUVECs were pretreated with PD98059 (10 μM), SP600125 (5 μM) or SB203580 (15 μM) for 1 h before PM stimulation. The expression levels of ICAM-1, VCAM-1 p62, LC3B and Bnip3 were detected using Western blotting. F HUVECs were pretreated with PD98059 (10 μM), SP600125 (5 μM) or SB203580 (15 μM) for 1 h before PM stimulation. Representative fluorescence images showing the adhesion of fluorescein-labeled THP-1 cells to HUVECs. *P < 0.05 compared with the HG + PM 0.5 h group; ^†P < 0.05 compared with the HG + PM group