Circulatory metabolites trigger ex vivo arterial endothelial cell dysfunction in population chronically exposed to diesel exhaust

Table 3 Internal dose of diesel exhaust exposure and plasma metabolites in 124 DETs and 123 non-DETs^a

Metabolite	Estimate	SE	Fold of change	Raw P	FDR
S-adenosylhomocysteine	− 0.080	0.016	0.92	5.7 × 10^–7	0.0001
3-Ketodihydrosphingosine	0.279	0.067	1.32	3.8 × 10^–5	0.0025
Isoleucine	− 0.017	0.004	0.98	5.6 × 10^–5	0.0025
IMP	− 0.244	0.062	0.78	0.0001	0.0037
Inosine	− 0.176	0.046	0.84	0.0002	0.0040
1-Methylhistidine	− 0.067	0.018	0.94	0.0002	0.0040
Carnitine C14	0.189	0.050	1.21	0.0002	0.0040
2-Aminooctanoic acid	0.136	0.038	1.15	0.0004	0.0062
Hypoxanthine	− 0.359	0.101	0.70	0.0005	0.0068
Indole-3-carboxylic acid	− 0.074	0.021	0.93	0.0005	0.0072
1-Methylnicotinamide	0.201	0.058	1.22	0.0006	0.0072
Carnitine C12	0.172	0.052	1.19	0.0010	0.0110
SDMA ADMA	− 0.051	0.016	0.95	0.0013	0.0132
Adenine	− 0.075	0.024	0.93	0.0016	0.0154
5'-Deoxy-5'-methylthioadenosine	− 0.089	0.029	0.91	0.0019	0.0169
N-Acetylaspartic acid	− 0.049	0.016	0.95	0.0021	0.0169
cGMP	− 0.092	0.030	0.91	0.0022	0.0172
Galactose	− 0.034	0.012	0.97	0.0035	0.0258
Asparagine	0.068	0.023	1.07	0.0040	0.0280
Carnitine C18	0.115	0.040	1.12	0.0043	0.0285
Acetylalanine	− 0.031	0.011	0.97	0.0058	0.0361
Serine	0.061	0.022	1.06	0.0067	0.0386

^aInternal dose of diesel exhaust exposure was defined as the urinary PC extracted from four urinary metabolites which has shown an excellent association with diesel exhaust exposure status. This PC was converted into an ordered categorical variable with three values (0, 1, 2) with each group having same number of subjects to calculate the estimate for the dose–response. Association between internal dose of diesel exhaust exposure and natural-log transformed serum metabolite levels was assessed using generalized linear model with adjustment for age, obesity, and internal dose for cigarette smoking. Fold of change was the exponential of the estimate

ISSN: 1743-8977