Circulatory metabolites trigger ex vivo arterial endothelial cell dysfunction in population chronically exposed to diesel exhaust

Table 5 The effect of cGMP on expression of individual biosensor genes from the exogenous cGMP addition experiment

Biosensor gene	Variable	Unit of change	Estimate (95%CI)^a	RQ^b	P^a
CCL2	cGMP (pmol/ml)	50	− 0.32 (− 0.62 to − 0.01)	1.25	0.043
CCL5	cGMP (pmol/ml)	50	− 1.53 (− 2.43 to − 0.63)	2.88	0.0025
VCAM	cGMP (pmol/ml)	50	− 0.45 (− 0.79 to − 0.12)	1.37	0.011

^aGeneralized linear model was used to quantify the change of expression (Delta Ct) of biosensor genes by addition of cGMP in the medium in exogenous cGMP addition experiment with adjustment for serum ID. The slopes of linear curves between gene expression (delta Ct) and cGMP levels were of no difference between serum samples from three individuals (all Ps > 0.07)
^bRelative quantification was calculated as RQ = 2^{−(estimate)}

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