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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Ultrasmall iron oxide nanoparticles cause significant toxicity by specifically inducing acute oxidative stress to multiple organs

Fig. 4

Identification of the generated ROS. a Fluorescence images of MCF-7 cells treated with different-sized Fe3O4, SiO2, and Au NPs and stained with DHR123 to characterize the generated H2O2. Green fluorescence: DHR123, an indicator of H2O2; blue fluorescence: Hoechst 33342. b The corresponding fluorescence intensities of DHR123 in MCF-7 cells after incubation with different NPs for 2 h and 6 h. c Generated O2− in cells determined by formazan formation using a WST-1 assay kit after incubation with NPs for 24 h. d Fluorescence intensities and e fluorescence images showing the generated ·OH in the cells using 3-CCA as probe. Green fluorescence: LysoGreen; blue fluorescence: 3-CCA, an indicator of ·OH. All of the data were presented as the mean ± SD (n = 3). P values: **P < 0.01, ***P < 0.001

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Particle and Fibre Toxicology

ISSN: 1743-8977

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