Fig. 2
Cytotoxicity screening of GO, MCLR and GO-MCLR in HaCaT and L02 cells. HaCaT cells and L02 cells, seeded at the density of 1 × 105 /mL, were respectively treated with GO (5 µg/mL and 50 µg/mL), MCLR (0.06 µg/mL and 0.6 µg/mL) and GO-MCLR (5 µg/mL + 0.06 µg/mL, 50 µg/mL + 0.6 µg/mL) for 24 h. a Use of a CCK-8 assay to assess the cells viability after exposure. The CCK-8 values in the control group was regarded as the 100% cell viability reference point. b LDH release amount representing the cell membrane integrity after exposure; c Cellular morphology of HaCaT and L02 cells under light microscope following exposure. *P < 0.05 versus Control. Green arrow: GO adhered to the cell surface; yellow arrow: the uptake of GO and GO-MCLR by cells; red arrow: cells rupture