Fig. 2

CDs induced different degrees of apoptosis and oxidative stress in two liver cell types. A and B are the distribution and quantification results of ROS in KUP5 and AML12 cells after CDs treatment at 0, 100, and 400 μg/mL for 24 h. N = 3 replications. C and D Scatterplot and quantification of KUP5 and AML12 cell apoptosis after treatment with 0, 100 and 400 μg/mL CDs for 24 h. Quantitative criterion was that the total apoptosis rate (Q1 + Q2) of the control group was lower than 10%. E–H Expression of apoptosis-related proteins Cleaved caspase3, Bax, and Bcl-2 in KUP5 and AML12 cells after CD treatment at 0, 100 and 400 μg/mL for 12 h. All values are mean ± SD. The statistical significance of differences was evaluated by one-way ANOVA. Compared with the control group. *, P < 0.05