Fig. 5

CDs resulted in different degrees of lysosomal damage in the two cell types. A Flow cytometry was used to analyze cell uptake of KUP5 and AML12 cells treated with 0 and 25 μg/mL CDs for 1 h. B Confocal microscopy was used to observe the distribution relationship between lysosomes and CDs in KUP5 and AML12 cells after 0 and 25 μg/mL CDs treatment for 1 h. LysoTracker Red is a lysosomal red fluorescent probe and blue is the distribution of CDs in cells. C, D Showed changes in the distribution and number of lysosomes labeled with LysoTracker Red in the treated and control groups. E Changes of lysosomal pH values in KUP5 and AML12 cells after 0 and 400 μg/mL CDs treatment for 1 h. LysoTracker Green DND is a lysosomal green fluorescent probe used to display changes in the acidic environment of the lysosome. N = 3 replications. All values are mean ± SD. The statistical significance of differences was evaluated by one-way ANOVA. Compared with the control group. *, P < 0.05