Fig. 7

Cell exposure to TiO₂ and CB nanoparticles promotes rise of Aβ42 through corruption of the PrP^C-PDK1 pathway. a Kinetics of intracellular Aβ accumulation in 1C11 cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) and related quantification histogram of immunostained Aβ. Scale bar = 10 µm. b ELISA-based quantification of Aβ42 peptides in 1C11 and 1C11^5−HT neuronal cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) up to 24 h. c ELISA-based quantification of Aβ42 peptides in 1C11^5−HT neuronal cells exposed to TiO₂- or CB-NPs (1 µg cm⁻²) for 4 h in the presence or not of a siRNA toward PrP^C (siPrP) or the PDK1 inhibitor, BX912 (1 µM). The experiments were performed three times in triplicates. Values are means ± SEM. *denotes p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus unexposed cells