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Table 2 Top 20 most abundant proteins for SHDS-treated and untreated FNPs

From: Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models

 

FNP SHDS + DMEM 10% FBS

FNP DMEM 10% FBS

 

LFQ ± SEM (x1E08)

Protein Name

Protein ID

MW (kDa)

LFQ ± SEM (x1E08)

Protein Name

Protein ID

MW (kDa)

1

12.4 ± 3.4

Bovine Alpha-2-HS-glycoprotein*

P12763

38.4

8.11 ± 1.6

Bovine Haemoglobin foetal subunit beta*

P02081

15.9

2

12.3 ± 1.8

Bovine Alpha-1-antiproteinase*

P34955

46.1

4.81 ± 0.19

Bovine Alpha-1-antiproteinase*

P34955

46.1

3

10.0 ± 0.90

Pig Peptidase S1 domain-containing protein**

I3LHI7

27.7

3.99 ± 0.050

Bovine Alpha-2-HS-glycoprotein*

P12763

38.4

4

7.96 ± 0.32

Bovine Albumin*

P02769

69.3

3.27 ± 0.34

Bovine Albumin*

P02769

69.3

5

6.84 ± 1.1

Bovine Haemoglobin foetal subunit beta*

P02081

15.9

3.22 ± 0.70

Bovine Haemoglobin subunit alpha*

P01966

15.2

6

4.03 ± 0.60

Bovine Apolipoprotein A-I*

P15497

30.3

2.67 ± 0.35

Bovine Apolipoprotein A-I*

P15497

30.3

7

3.42 ± 0.76

Bovine Histone H2A type 2-C

A1A4R1

14

2.45 ± 0.11

Bovine Inter-alpha-trypsin inhibitor heavy chain H2*

A0A3Q1LK49

96.8

8

2.45 ± 0.24

Bovine Haemoglobin subunit alpha*

P01966

15.2

2.35 ± 0.35

Bovine Angiotensinogen*

P01017

51.4

9

2.43 ± 0.35

Pig Triacylglycerol lipase**

F1S4T9

51.6

2.04 ± 0.18

Bovine Alpha-fetoprotein*

Q3SZ57

68.6

10

2.42 ± 0.35

Pig HATPase_c domain-containing protein**

A0A287A9T4

83

1.90 ± 0.16

Bovine Apolipoprotein A-II*

P81644

11.2

11

2.32 ± 0.57

Bovine Serpin family G member 1

E1BMJ0

51.8

1.88 ± 0.14

Bovine Inter-alpha-trypsin inhibitor heavy chain H4

F1MMD7

101.5

12

2.26 ± 0.42

Pig Carboxypeptidase A1**

P09954

47.2

1.87 ± 0.10

Bovine Alpha-1-microglobulin

F1MMK9

53

13

2.02 ± 0.80

Bovine Angiotensinogen*

P01017

51.4

1.80 ± 0.17

Bovine Beta-2-glycoprotein 1*

P17690

38.3

14

2.00 ± 0.13

Bovine Alpha-fetoprotein*

Q3SZ57

68.6

1.54 ± 0.090

Bovine Alpha-2-macroglobulin

Q7SIH1

167.6

15

1.61 ± 0.85

Bovine Histone H2A

F2Z4G5

14.1

1.52 ± 0.15

Bovine Fetuin-B

Q58D62

42.7

16

1.58 ± 0.10

Bovine Beta-2-glycoprotein 1*

P17690

38.3

1.46 ± 0.24

Bovine Vitronectin*

Q3ZBS7

53.6

17

1.58 ± 0.10

Bovine Inter-alpha-trypsin inhibitor heavy chain H2*

A0A3Q1LK49

96.8

1.46 ± 0.037

Bovine Complement C3

Q2UVX4

187.3

18

1.39 ± 0.16

Bovine Apolipoprotein A-II*

P81644

11.2

1.39 ± 0.12

Bovine Alpha-1B-glycoprotein

Q2KJF1

53.6

19

1.30 ± 0.19

Pig Peptidase S1 domain-containing protein**

I3LJ52

26.9

1.28 ± 0.11

Bovine Inter-alpha-trypsin inhibitor heavy chain H3

P56652

99.6

20

1.14 ± 1.7

Bovine Vitronectin*

Q3ZBS7

53.6

1.28 ± 0.11

Bovine Complement factor B

P81187

85.4

  1. *Common proteins; **proteins from SHDS. LFQ was calculated with Perseus (n = 3). SEM refer to the standard error of the mean for n = 3
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Particle and Fibre Toxicology

ISSN: 1743-8977

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