Table 5 Sequences of qRT-PCR primers
Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
TJP1 | CCCCACTCTGAAAATGAGGA | ACAGCAATGGAGGAAACAGC |
OCLN | ATGCCATGGGACTGTCAACT | TTTGTGGGACAAGGAACACA |
CLDN3 | CCTGCGTCTGTCCCTTAGAC | CACGCGAGAAGAAGTACACG |
CLDN5 | GCTGTTTCCATAGGCAGAGC | CCCTGCCGATGGAGTAAAGA |
TNF | TGGGATCATTGCCCTGTGAG | GGTGTCTGAAGGAGGGGGTA |
IL6 | GGTACATCCTCGACGGCATCT | GTGCCTCTTTGCTGCTTTCAC |
IL10 | AGACAGACTTGCAAAAGAAGGC | TCGAAGCATGTTAGGCAGGTT |
IL22 | GCTGCCTCCTTCTCTTGG | GTGCGGTTGGTGATATAGG |
S14 | AGGTGCAAGGAGCTGGGTT | TCCAGGGGTCTTGGTCCTATTT |