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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Macrophage-mediated tissue response evoked by subchronic inhalation of lead oxide nanoparticles is associated with the alteration of phospholipases C and cholesterol transporters

Fig. 4

Detection of Pb content in lung nanoparticles by EDS. A, B STEM images of ultrathin sections collected with brightfield detector (A) with detecting angle 9.8 mrad and HAADF detector (B) detecting angle of 24.4–89.4 mrad. Alveolar macrophage (ma) with cytoplasmic processes (pr) and phagosomes (ph) with nanoparticles (arrowheads) in lung tissue after PbO NP inhalation. Cyan rectangles on (B) outline areas from which EDS spectra were recorded with ROI 1 being empty resin, ROI 2 being cytoplasm without any dense objects and NPS 1 and 2 marking phagosomes with nanoparticles. C, D EDS spectra with spectrum of cytoplasm (ROI 2) in red and spectra of NPS 1 and 2, respectively, in blue. Inserts show details of measured spectra with rectangles marking major peaks Pb Ma at 2.34 keV and Pb La at 10.55 keV. E Table summarizes estimates of relative mass of different elements in outlined regions of interest based on standard-less quantitative analysis

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Particle and Fibre Toxicology

ISSN: 1743-8977

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