Fig. 11

DNA damage in AEC2. Representative immunohistochemical staining images of the Ser-139 residue of the histone variant H2AX (γ-H2AX), a DNA double-strand break marker, and LPCAT1, an AEC2 marker (A). The γ-H2AX -positive index in AEC2 was calculated as the percentage of γ-H2AX and LPCAT1 double positive cells compared to the total LPCAT1-positive cell population (B) (n = 5). Statistical significance was analyzed using two-way ANOVA with Tukey’s multiple comparison test: *p < 0.05 and ***p < 0.001 indicate significant differences