Biokinetics of subacutely co-inhaled same size gold and silver nanoparticles

Table 3 The organ distribution for silver after inhaled of AgNP at exposure 1 day (E-1), post-exposure observation 1 day (PEO-1), PEO-7 days and PEO-28 days (mean ± S.E; 4 animals of exposure 1 day; 5 animals of PEO-1, PEO-7 and PEO-28). A statistics analysis was used Anova and test

AgNP	Liver			Kidney			Spleen			Testis			Epididymis
AgNP	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B
E-1	7.981 ± 1.458	8.767 ± 0.332	69.407 ± 11.214	0.767 ± 0.223	1.175 ± 0.061	0.926 ± 0.327	N/D	0.657 ± 0.131	N/D	0.261 ± 0.130	1.488 ± 0.174	0.344 ± 0.142	N/D	0.339 ± 0.022	N/D
PEO1	7.235 ± 1.907	9.418 ± 0.467	66.974 ± 18.033*	0.486 ± 0.266	1.192 ± 0.043	0.623 ± 0.376	0.724 ± 0.493	0.600 ± 0.013	0.426 ± 0.118	0.284 ± 0.083	1.511 ± 0.086	0.431 ± 0.126	0.166 ± 0.030	0.506 ± 0.035	0.078 ± 0.014^a
PEO7	0.215 ± 0.149	10.717 ± 0.430	2.573 ± 1.593	0.444 ± 0.444	1.330 ± 0.053	0.575 ± 0.575	1.279 ± -	0.723 ± 0.040	0.836 ± -	0.195 ± 0.082	1.550 ± 0.108	0.257 ± 0.112	0.641 ± 0.218	0.624 ± 0.065	0.359 ± 0.112^aa
PEO 28	N/D	11.768 ± 0.180	N/D	N/D	1.449 ± 0.056	N/D	N/D	0.972 ± 0.068	N/D	0.168 ± 0.168	1.677 ± 0.046	0.272 ± 0.272	0.102 ± 0.010	0.610 ± 0.047	0.062 ± 0.008

Co-exposure	Liver			Kidney			Spleen			Testis			Epididymis
E-1	2.238 ± 0.628	9.362 ± 1.021	22.598 ± 8.612	0.198 ± -	1.226 ± 0.110	0.244 ± -	N/D	0.589 ± 0.074	0.166 ± 0.044	N/D	1.528 ± 0.044	N/D	N/D	0.296 ± 0.012	N/D
PEO1	4.912 ± 1.988	9.237 ± 0.529	42.262 ± 14.830	0.372 ± 0.155	1.257 ± 0.037	0.469 ± 0.190	1.832 ± 0.197	0.571 ± 0.023	0.999 ± 0.099	0.199 ± 0.050	1.638 ± 0.089	0.313 ± 0.069	0.008 ± 0.008	0.482 ± 0.058	0.004 ± 0.004
PEO7	0.894 ± 0.673	9.987 ± 0.919	7.098 ± 4.499	0.375 ± -	1.300 ± 0.074	0.537 ± -	0.699 ± 0.111	0.656 ± 0.043	0.492 ± 0.126	0.279 ± 0.061	1.611 ± 0.087	0.476 ± 0.125	0.235 ± 0.077	0.550 ± 0.051	0.125 ± 0.046
PEO 28	N/D	13.905 ± 0.894	N/D	N/D	1.609 ± 0.053	N/D	N/D	1.054 ± 0.142	N/D	0.132 ± -	1.677 ± 0.021	0.211 ± -	0.327 ± 0.070	0.675 ± 0.080	0.213 ± 0.076

AgNP	Thymus			Olfactory bulb			Hilar lymph node			Brachial lymph node			Brain
AgNP	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B	Au/organ (ng)^C	Au/organ (ng)^C	ng/g^A	Organ wt (g)^B
E-1	N/D	0.597 ± 0.050	N/D	2.842 ± 1.175	0.044 ± 0.008	0.098 ± 0.030	62.109 ± 8.273	0.004 ± 0.001	0.206 ± 0.039	3.009 ± 1.858	0.044 ± 0.008	0.095 ± 0.048	N/D	2.093 ± 0.015	N/D
PEO1	N/D	0.471 ± 0.098	N/D	25.646 ± 4.401	0.059 ± 0.002	1.552 ± 0.303	16.337 ± 13.087	0.014 ± 0.003	0.196 ± 0.157	8.119 ± 4.437	0.059 ± 0.002	0.495 ± 0.279	1.219 ± 0.151	2.049 ± 0.061	2.507 ± 0.336
PEO7	0.482 ± -	0.497 ± 0.031	0.280 ± -	24.925 ± 3.328	0.063 ± 0.003	1.559 ± 0.200	4.055 ± 1.298	0.042 ± 0.006	0.146 ± 0.044	18.072 ± 9.080	0.063 ± 0.003	1.152 ± 0.504	0.941 ± 0.121	2.192 ± 0.040	2.045 ± 0.231
PEO 28	N/D	0.393 ± 0.034	N/D	19.160 ± 0.567	0.065 ± 0.004	1.245 ± 0.108	N/D	0.033 ± 0.006	N/D	7.073 ± 5.843	0.065 ± 0.004	0.387 ± 0.306	1.195 ± 0.115	2.147 ± 0.046	2.568 ± 0.262

Co-exposure	Thymus			Olfactory bulb			Hilar lymph node			Brachial lymph node			Brain
E-1	0.146 ± -	0.573 ± 0.043	0.146 ± -	1.180 ± 0.394	0.061 ± 0.008	0.065 ± 0.026	15.323 ± 6.319	0.019 ± 0.005	0.105 ± 0.068	N/D	0.061 ± 0.008	N/D	N/D	2.093 ± 0.015	N/D
PEO1	0.958 ± 0.279	0.430 ± 0.055	0.277 ± 0.044	9.369 ± 2.395	0.103 ± 0.008	0.916 ± 0.192^b	11.537 ± 6.950	0.022 ± 0.005	0.143 ± 0.079	0.192 ± -	0.103 ± 0.008	0.018 ± -	0.284 ± 0.105	2.079 ± 0.023	0.593 ± 0.221
PEO7	0.252 ± -	0.498 ± 0.044	0.152 ± -	4.443 ± 0.786	0.108 ± 0.008	0.484 ± 0.105	0.242 ± -	0.034 ± 0.004	0.004 ± -	0.303 ± -	0.108 ± 0.008	0.035 ± -	0.752 ± 0.280	2.118 ± 0.062	1.536 ± 0.553
PEO 28	N/D	0.466 ± 0.044	N/D	3.302 ± 0.378	0.111 ± 0.008	0.372 ± 0.061	N/D	0.050 ± 0.005	N/D	N/D	0.111 ± 0.008	N/D	0.510 ± 0.151	2.277 ± 0.032	1.144 ± 0.322

A, samples were analyzed using by ICP-MS and calculated following ppb (ug/L) x final volume (5 mL) x dilution factor (10 times) / organ weight.
B, Organ weights were measured using by micro balance. Before measure weight, washed blood to di-water and wiped up to paper.
C, The silver concentration (ng/g) x organ weight (g)
N/D was analyzed sample silver concentration lower than compare with control.
A statistics were analyzed Anova (multiple comparison analysis of Dennett test) and t-test. This result was described in Table that Anova was from a to cc and t-test was * and **.
Where (Anova)
^aP < 0.05, compared with PEO-1 and PEO-7
^bP < 0.05, compared with PEO-1 and PEO-28
^aaP < 0.01, compared with PEO-7 and PEO-28
Where (t-test)
*P < 0.05, and **P < 0.01

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