Fig. 3

Enhancement of microglial activation during ischemia induced by CRM28 exposure. CRM28 was suspended in water and intranasally (i.n.) administered at doses of 0, 10 or 100 µg/mouse once a day for 7 days. One day after induction of photothrombosis, brain slices were prepared and stained with Iba1 and CD68. The peripheral area of the ischemic area in the cerebral cortex was observed with a confocal microscope. A Representative stained pictures of the cerebral cortex. B Amoeboid scores. C The CD68-stained area of the cerebral cortex. The values are presented as the mean ± S.D. (n = 6 animals in each group). The data between vehicle and vehicle + PT1d were analyzed using Student’s t test. The data among vehicle + PT1d, 10 µg + PT1d and 100 µg + PT1d were analyzed using one-way ANOVA [B: F(2, 15) = 12.57, p = 0.0006, C: F(2, 15) = 9.223, p = 0.0024] followed by Dunnett’s multiple comparisons test