Oral toxicological study of titanium dioxide nanoparticles with a crystallite diameter of 6 nm in rats

Akagi, Jun-ichi; Mizuta, Yasuko; Akane, Hirotoshi; Toyoda, Takeshi; Ogawa, Kumiko

doi:10.1186/s12989-023-00533-x

Particle and Fibre Toxicology

Table 8 Organ weights of F344/DuCrlCrlj rats treated with titanium dioxide nanoparticles for 90 days

From: Oral toxicological study of titanium dioxide nanoparticles with a crystallite diameter of 6 nm in rats

Dose (mg/kg bw/day)	0	100	300	1000
Male
No. of animals	10	10	10	10
Body weight (g)	291.4 ± 16.4	285.8 ± 17.0	297.3 ± 16.8	284.4 ± 16.7
Absolute
Brain (g)	1.911 ± 0.046	1.914 ± 0.034	1.916 ± 0.030	1.902 ± 0.035
Thymus (g)	0.168 ± 0.016	0.162 ± 0.028	0.182 ± 0.018	0.159 ± 0.026
Lungs (g)	0.906 ± 0.042	0.875 ± 0.061	0.923 ± 0.057	0.893 ± 0.061
Heart (g)	0.829 ± 0.058	0.829 ± 0.042	0.851 ± 0.039	0.833 ± 0.054
Spleen (g)	0.570 ± 0.043	0.569 ± 0.049	0.583 ± 0.035	0.553 ± 0.035
Liver (g)	6.553 ± 0.644	6.400 ± 0.468	6.649 ± 0.399	6.183 ± 0.501
Adrenals (g)	0.035 ± 0.004	0.034 ± 0.003	0.034 ± 0.003	0.035 ± 0.003
Kidneys (g)	1.578 ± 0.076	1.622 ± 0.104	1.613 ± 0.086	1.586 ± 0.098
Testes (g)	2.944 ± 0.116	2.964 ± 0.293	2.912 ± 0.268	2.949 ± 0.346
Pituitary (mg)	6.61 ± 0.83	6.68 ± 0.80	7.12 ± 0.77	6.91 ± 0.75
Thyroid (mg)	14.63 ± 1.61	12.59 ± 2.44	13.87 ± 2.69	12.33 ± 1.84
Salivary gland (g)	0.453 ± 0.033	0.448 ± 0.026	0.465 ± 0.018	0.446 ± 0.030
Seminal vesicle (g)	0.844 ± 0.122	0.804 ± 0.097	0.897 ± 0.093	0.830 ± 0.087
Prostate gland (g)	0.638 ± 0.082	0.667 ± 0.064	0.696 ± 0.072	0.680 ± 0.071
Relative (%)
Brain	0.659 ± 0.035	0.672 ± 0.039	0.646 ± 0.031	0.671 ± 0.035
Thymus	0.059 ± 0.005	0.056 ± 0.008	0.061 ± 0.008	0.056 ± 0.010
Lungs	0.312 ± 0.017	0.340 ± 0.109	0.311 ± 0.014	0.314 ± 0.018
Heart	0.284 ± 0.009	0.290 ± 0.008	0.286 ± 0.011	0.293 ± 0.015
Spleen	0.196 ± 0.014	0.199 ± 0.008	0.196 ± 0.008	0.194 ± 0.007
Liver	2.245 ± 0.107	2.238 ± 0.046	2.236 ± 0.029	2.172 ± 0.073
Adrenals	0.012 ± 0.001	0.012 ± 0.001	0.011 ± 0.001	0.012 ± 0.001
Kidneys	0.538 ± 0.026	0.568 ± 0.022 **	0.543 ± 0.011	0.558 ± 0.019
Testes	1.012 ± 0.054	1.039 ± 0.099	0.981 ± 0.084	1.036 ± 0.085
Pituitary	0.002 ± 0.000	0.002 ± 0.000	0.002 ± 0.000	0.002 ± 0.000
thyroid	0.005 ± 0.001	0.004 ± 0.001	0.005 ± 0.001	0.004 ± 0.001
Salivary gland	0.155 ± 0.007	0.157 ± 0.005	0.157 ± 0.008	0.157 ± 0.009
Seminal vesicle	0.290 ± 0.042	0.281 ± 0.029	0.303 ± 0.038	0.292 ± 0.030
Prostate gland	0.219 ± 0.024	0.234 ± 0.020	0.235 ± 0.027	0.240 ± 0.027
Female
No. of animals	10	10	10	10
Body weight (g)	173.0 ± 5.3	171.1 ± 5.3	170.3 ± 7.1	169.4 ± 7.1
Absolute
Brain (g)	1.796 ± 0.028	1.787 ± 0.031	1.779 ± 0.030	1.784 ± 0.037
Thymus (g)	0.158 ± 0.015	0.152 ± 0.018	0.147 ± 0.008	0.154 ± 0.015
Lungs (g)	0.676 ± 0.029	0.694 ± 0.036	0.674 ± 0.134	0.686 ± 0.039
Heart (g)	0.555 ± 0.018	0.548 ± 0.032	0.554 ± 0.030	0.540 ± 0.027
Spleen (g)	0.386 ± 0.019	0.385 ± 0.013	0.372 ± 0.023	0.374 ± 0.027
Liver (g)	3.751 ± 0.122	3.693 ± 0.149	3.640 ± 0.182	3.624 ± 0.176
Adrenals (g)	0.040 ± 0.003	0.041 ± 0.004	0.039 ± 0.006	0.040 ± 0.006
Kidneys (g)	1.021 ± 0.057	1.027 ± 0.032	1.000 ± 0.051	1.001 ± 0.041
Ovaries (g)	0.049 ± 0.006	0.045 ± 0.009	0.044 ± 0.006	0.043 ± 0.007
Pituitary (mg)	11.49 ± 2.02	11.72 ± 1.33	10.58 ± 1.97	11.44 ± 1.92
Thyroid (mg)	10.95 ± 2.36	9.54 ± 0.94	11.35 ± 1.35	11.07 ± 1.12
Salivary gland (g)	0.326 ± 0.012	0.335 ± 0.011	0.333 ± 0.018	0.333 ± 0.023
Relative (%)
Brain	1.039 ± 0.029	1.045 ± 0.028	1.047 ± 0.048	1.055 ± 0.061
Thymus	0.091 ± 0.007	0.089 ± 0.009	0.087 ± 0.005	0.091 ± 0.008
Lungs	0.391 ± 0.014	0.406 ± 0.023	0.396 ± 0.076	0.405 ± 0.026
Heart	0.321 ± 0.016	0.321 ± 0.016	0.325 ± 0.013	0.319 ± 0.013
Spleen	0.223 ± 0.009	0.225 ± 0.009	0.218 ± 0.011	0.221 ± 0.020
Liver	2.169 ± 0.063	2.160 ± 0.095	2.138 ± 0.061	2.140 ± 0.092
Adrenals	0.023 ± 0.001	0.024 ± 0.003	0.023 ± 0.003	0.023 ± 0.004
Kidneys	0.590 ± 0.029	0.601 ± 0.020	0.587 ± 0.020	0.592 ± 0.029
Ovaries	0.028 ± 0.003	0.026 ± 0.005	0.026 ± 0.003	0.025 ± 0.004
Pituitary	0.007 ± 0.001	0.007 ± 0.001	0.006 ± 0.001	0.007 ± 0.001
Thyroid	0.006 ± 0.001	0.006 ± 0.001	0.007 ± 0.001	0.007 ± 0.001
Salivary gland	0.189 ± 0.006	0.196 ± 0.009	0.195 ± 0.008	0.197 ± 0.010

Values are mean ± S.D
**P < 0.01, compared with the 0 mg/kg bw/day group

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