Fig. 5

The transcriptional response upon DEP involves inflammatory and interferon responses in colon macrophages. A Principal component analysis of colon macrophages from diesel exhaust particles (DEP) or PBS-exposed mice, showing Ccr2⁺ and Ccr2⁻ subpopulations (colors represent clusters; contours cell density) and relative proportion across clusters. B Differential 2D density plot (red indicates excess of DEP-, blue excess of PBS-exposed cells). C Cluster-specific differential gene expression (closed circles indicate genes related to interferon regulation, open circles genes related to macrophage activation, half-open circles to both). D Significantly up-regulated MSigDB Hallmark pathways upon DEP exposure. One experiment with n = 2. DEP: Diesel exhaust particles, FDR: false discovery rate, PBS: Phosphate-buffered saline