Fig. 4

Western blotting of cell signaling mediators in the lungs of WT and Par2 KO mice exposed to HDM extract and MWCNTs. A Western blots of whole lung lysates from all animals evaluated (see Additional file 6: Fig. S5 for the original uncropped western blots). B Densitometry of p-SMAD2/3 to total SMAD2/3. C Densitometry of p-STAT6 normalized against total STAT6. D Densitometry of p-NF-κB normalized against total NF-κB. E Densitometry of ARG-1 against β-Actin. n = 5. ****p < 0.0001, **p < 0.01 when compared to respective vehicle control groups, ^^^^p < 0.0001, ^^p < 0.01 when compared to respective HDM groups, ####p < 0.0001, ##p < 0.01 when compared to respective MWCNT groups, &&p < 0.01, &p < 0.05 between genotypes as determined by two-way ANOVA with Tukey’s post hoc analysis