Fig. 7
MLE-12 endocytosis of highly expressed Gpnmb EVs promotes the EMT process. A. Representative western blot results showing higher GPNMB expression in EVs in the SiO2 group than in the control group. B. Images of EVs incubated with MLE-12 for 0, 6, 12, 24, and 48 h. C. Representative western blot results showing elevated protein levels of COL1, FN1, and α-SMA in the SiO2-EVs group compared to the control group. D. *P < 0.05 indicates higher COL1 protein levels in the SiO2-EVs group compared to the control group, while #P < 0.05 indicates higher FN1 protein levels in the SiO2-EV group compared to the control group. E. Representative western blot results showing elevated expression of GPNMB in EVs secreted by cells in the Gpnmb-OE group compared to the Gpnmb-NC group. F. *P < 0.05 indicates that the GPNMB protein levels were higher in the Gpnmb-OE group than in the Gpnmb-NC group. G. Representative western blot results showing elevated protein levels of COL1, FN1, and α-SMA in the Gpnmb-OE group compared to the control group. H. *P < 0.05 indicates that the α-SMA protein level was higher in the Gpnmb-OE group compared to the control group; #P < 0.05 indicates that the COL1 protein level was higher in the Gpnmb-OE group compared to the control group; and &P < 0.05 indicates that the FN1 protein level was higher in the Gpnmb-OE group compared to the control group