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Fig. 8 | Particle and Fibre Toxicology

Fig. 8

From: Amorphous silica nanoparticles cause abnormal cytokinesis and multinucleation through dysfunction of the centralspindlin complex and microfilaments

Fig. 8

ROS inhibitor NAC attenuated the inhibition of PI3K 110β/Aurora B pathway and cytokinesis related regulation proteins induced by aSiNPs. (A) Results of western blot analysis. (B) Relative densitometric analysis of the protein bands (a-g) and detection of Rho activity (h) were performed and presented. (a) PI3K 110β, (b) p-Aurora B, (c) MKLP1, (d) CYK4, (e) Ect2, (f) Cep55, (g) CHMP2A, (h) Rho activity detected by G-LISA biochem kit. (C) Relative ratio of CYK4 bound to MKLP1 was detected by co-immunoprecipitaion to reflect the formation of centralspindlin cluster. Data were expressed as means ± SD from three independent experiments. * p < 0.05 compared with control group using one-way ANOVA.

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Particle and Fibre Toxicology

ISSN: 1743-8977

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