Fig. 5
From: Long-term exposure to polystyrene microplastics triggers premature testicular aging
PS-MPs exposure caused premature senescence of TM4 cells. a Box plot showing cell viability after PS-MPs exposure for 24 h. b Representative fluorescent images of SA-β-Gal staining in TM4 cells. c Quantitative analysis of SA-β-Gal staining as presented in b. d Western blot of p21, p16, p53 and H3K9me3 in TM4 cells treated with control and PS-MPs. e-h Quantification of protein levels of p21, p16, p53 and H3K9me3 described in d. β-actin and Histone considered as loading control. i, k Flow cytometry showing apoptotic cells and percentage of cell cycle. j qPCR analysis of Caspase 3. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm