Fig. 8

The effect of PS-MPs exposure on inflammatory response. a Western blot of NF-κB, IL-6, IL-8 and TNF-α in TM4 cells treated with control and PS-MPs. b-e Quantification of protein levels of NF-κB, IL-6, IL-8 and TNF-α described in a. β-actin considered as loading control. f Detection of ROS levels in TM4 cells after treated with PS-MPs and PS-MPs + NAC. g Quantitative analysis of fluorescent intensity in f. h, j Western blot showing the expression of p-NF-κB in TM4 cells after treated with PS-MPs and PS-MPs + NAC (H) or PS-MPs + BAY 11-7082 j. i Quantification of protein levels of p-NF-κB described in h, j. l Representative fluorescent images showing SA-β-Gal activity treated with PS-MPs and PS-MPs + BAY 11-7082. m Quantitative analysis of fluorescent intensity of SA-β-Gal described in l. n Schematic diagram of PS-MPs causing premature TM4 cellular senescence through ROS and NF-κB mediation. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm