Fig. 2
TiO2 NP exposure disrupted the placental barrier function. a, b We collected images with an in vivo imaging system (IVIS) after the indocyanine green injection. It showed stronger ICG signals in the hypogastrium in pregnant mice from the 50 and 250 mg/kg groups. Normalized by the signal intensity in the feet, the hypogastrium intensities increased in a dose-dependent manner. c Significant decreases of Ocln and Tjp gene expression were observed following TiO2 exposure, suggesting disrupted placenta barrier function. Data are presented as mean ± SEM. n = 3 mice in IVIS; n = 7 placentas from 7 mice in qPCR. *p < 0.05 and **p < 0.01 as compared with the normal control (NC)