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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model

Fig. 3

Surfactant production and macrophage viability at the air-liquid interface (ALI). Co-cultures of A549 cells with wild type (WT), CASP1−/−, and NLRP3−/− THP-1 cells as well as A549 mono-cultures were fixed after 48 h of cultivation at the ALI. Nuclei were stained with Hoechst 33342. Immunostaining of surfactant protein C (SP-C) and CD45 was performed. Representative images were obtained at 100 x magnification

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