Fig. 4From: Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture modelDQ12 quartz dust and Saharan dust were not cytotoxic. Air-liquid interface (ALI) co-cultures were exposed to DQ12 and Saharan dust (SD) at doses in the range of 0–31 µg/cm² for 24 h or to 250 ± 60 ng/cm² LPS for 21 h. The apical (A) and basolateral (B) release of adenylate kinase (AK) was examined as a marker of cytotoxicity using the ToxiLight assay. ALI cultures treated with 0.5% Triton X-100 served as lysis control. The values are plotted as means ± standard deviation of N = 4 independent experiments (Controls and LPS: one or two biological replicates; DQ12 and SD; duplicates per concentration). A mixed-effects model with Šidák’s post hoc test was calculated (no significant differences obtained)Back to article page