Fig. 6
High glucose pretreatment enhanced the effects of PM2.5 on the activation of NLRP3 inflammasome, but not TLR2, in MH-S cells. The cells were treated with 50 μg/mL of PM2.5 for 6 h (a and b) or 24 h (d) with/without 30 mM of glucose pretreatment for 18 days. c The cells were treated with 50 μg/mL of PM2.5 for 6, 12, and 24 h. The cells treated with physiological saline were used as control. a is the Western blot result of a single experiment while b is the normalized results of Western blots. c and d The cell culture media were collected for ELISA by Mouse IL-1beta ELISA kit (Invitrogen). Data are shown as mean ± SEM (n = 3). * p < 0.05 vs. control; # p < 0.05 vs. group with PM2.5 treatment only. n.s., not specific