Fig. 7
Enhanced NF-κB nuclear translocation in MH-S cells exposed to PM2.5 in a high glucose environment. The cells were treated with 25, 50, and 100 μg/mL of PM2.5 for 6 h (a and b) or with 50 μg/mL of PM2.5 for 3, 6, and 12 h (c and d). e and f The cells were treated with 50 μg/mL of PM2.5 for 6 h with/without 30 mM glucose pretreatment for 18 days. The cells treated with physiological saline were used as control. Nuclear and cytoplasmic proteins were extracted from the cells by using NE-PER™ Nuclear and Cytoplasmic Extraction Reagent (Thermo Scientific). The expression of β-actin was used as an internal reference for cytoplasmic protein while histone H3 was for nuclear protein. a, c, e are Western blot results of a single experiment while b, d, f are normalized results of Western blots. Data are shown as mean ± SEM (n = 3). * p < 0.05 vs. control; # p < 0.05 vs. group with PM2.5 treatment only. Nuc, nuclear protein; Cyt, cytoplasmic protein