High glucose enhances the activation of NLRP3 inflammasome by ambient fine particulate matter in alveolar macrophages

Table 1 Primers used for RT-qPCR

Gene	Forward (5’ → 3’)	Reverse (5’ → 3’)	Product size
IL-1β	TCATGGGATGATGATGATAACCTGCT	CCCATACTTTAGGAAGACACGGATT	503 bp
IL-18	GACTCTTGCGTCAACTTCAAGG	CAGGCTGTCTTTTGTCAACGA	169 bp
NLRP3	GCAGGAGGAAGACTTTGTGC	AGGAGATGTCGAAGCAGCAT	461 bp
Caspase-1	TACCTGGCAGGAATTCTGGA	ATGATCACCTTGGGCTTGTC	596 bp
TLR2	ACAGCTACCTGTGTGACTCTCCGCC	GGTCTTGGTGTTCATTATCTTGCGC	602 bp
TLR4	GCTTTCACCTCTGCCTTCAC	AGGCGATACAATTCCACCTG	259 bp
MMP-2	CCAACTACGATGATGAC	ACCAGTGTCAGTATCAG	233 bp
MMP-9	ACCACCACAACTGAACCACA	ACCAACCGTCCTTGAAGAAA	304 bp
TIMP-1	ACCACCTTATACCAGCGTTA	AAACAGGGAAACACTGTGCA	305 bp
TIMP-2	CACCCGCAACAGGCGTTTTG	ATCTTGCCATCTCCTTCTGC	269 bp
β-actin	GGCATTGTTACCAACTGGGAC	ACCAGAGGCATACAGGGACAG	219 bp

ISSN: 1743-8977