Fig. 4

PS-NP-induced aberrant mitophagy mediated by PINK1/Parkin in differentiated SH-SY5Y cells. A-D The quantification of mitophagy-related proteins, including LC3-II/LC3-I, p62, PINK1, and Parkin. E Representative IF images showing colocalization of LC3 (green) and Mito tracker (red). F-I Differentiated SH-SY5Y cells were exposed to 500 μg/mL PS-NPs with or without 3-MA (100 μM) for 48 h. The quantification of protein expression levels of (F) LC3-II/LC3-I and (G) p62. (H) Cell viability and (I) ATP levels. Results are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, compared to the 0 μg/mL group or indicative group