Table 1 Primer sequences used for real-time qPCR
Gene | Direction | Primer sequence (5’-3’) | Tm(°C) |
|---|---|---|---|
β-actin TNF-α IL-10 | Forward Reverse Forward Reverse Forward Reverse | ACTGCCGCATCCTCTTCCTC AAAGAGCCTCAGGGCATCGG TAGCCCACGTCGTAGCAAAC GCAGCCTTGTCCCTTGAAGA AAGGGTTACTTGGGTTGCCA CCTGGGGCATCACTTCTACC | 59.5 59.5 59.5 59.5 59.5 59.5 |