Fig. 8

ROS/mtROS regulates TNTs formation under nanomaterials exposure via PI3K/AKT/mTOR pathway in U251 cells. Cells were pretreated with 10 mM NAC or 0.2 μM MitoQ for 30 min and then exposed to nanomaterials for 24 h. A Western blot analysis of the PI3K/AKT/mTOR pathway in U251 cells. B–H Statistic analysis of PI3K/AKT/mTOR pathway in U251 cells. *, ** and *** indicate P < 0.05, 0.01 and 0.01 compared with control group. ^# and ^## indicate P < 0.05 and 0.01 compared with the respective nanomaterials group (regarding the no intervention nanomaterials group). Date present as mean ± SEM. n = 3