Fig. 3

Internalization of serum-coated MNPs by THP-1-derived macrophages after 1 h of exposure. Cells were exposed to 125 µg/ml of serum-coated MNPs A PS50, B PS100, C PS100 (+), D PS100 (−), E PS200, F PS500 and g PS1000. At the side of each image, the orthogonal Y–Z and at the bottom, the orthogonal X–Z views are shown, which represent slices through the Y–Z and X–Z planes. The yellow cross in the X–Y image indicates the origin of the orthogonal images. The red stain corresponds to the wheat germ agglutinin cell surface coating, and the green stain corresponds to the fluorescent PS MNPs