Fig. 8

Differential abundance of proteins between serum-coated and digested+serum-coated MNPs. Differential abundance of proteins in serum-coated vs. digested+serum-coated PS MNPs A PS100, B PS100 (+), C PS100 (−), D PS1000 MNPs. Every dot represents a unique protein identified. The X-axis represents the log2 (fold change) between the serum-coated and digested+serum-coated MNPs. The y-axis indicates the -log of the P value. The black lines indicate the P value threshold and fold-change threshold used to consider a protein as differentially abundant. Colored dots are differentially abundant, while dots in gray are considered equal between treatments. A negative log2-fold change (red dots) represents proteins that are more abundant in the digested+serum-coated protein corona, while a positive log2-fold change (blue dots) represents proteins more abundant in the serum-coated protein corona. Differential expression testing was done by multiple T-tests with permutation based FDR correction at FDR = 0.05. Proteins with an absolute log2fold difference larger than 2 and a P-value below 0.05 were considered significantly differentially abundant