Fig. 2

EN590-PM and CNG-PM are not cytotoxic. a and b Representative images of iMGLs treated with vehicle, 10–300 µg ml⁻¹ EN-PM and CNG-PM, or positive control (PC: 400 µM 1-methyl-4-phenylpyridinium) at 24 h (top) and 48 h (bottom) with Cytotox Green Reagent labeling dead cells. Scale bar 100 µm. Cell lines: MBE, MAD6. c Time curve for Cytotox green positive dead cell count per cell density for vehicle and 10–100 µg ml⁻¹ EN-PM treated iMGLs live-imaged for 48 h and normalized to PC at 48 h. Cell lines: MBE, MAD6. d Respective bar graphs for area under curves (AUC). Cell lines: MBE, MAD6. e Cell death assessed with the LDH test and f metabolic activity assessed with MTT test after 24-h exposure. Corresponding g Cytotox time-curve, h AUC, Cell lines: 2 × MBE, MAD6., i LDH and j MTT quantification for 10–300 µg ml⁻¹ CNG-PM treated iMGLs. Cell lines: 3 × BIONi. c and d n = 2 experiments each with 3 wells. e–j n = 2–3 experiments each with 3–6 wells. Data as mean ± SEM. Non-parametric Kruskal–Wallis test followed by Dunn’s test. See also Fig. 3