Fig. 4

EN590- and CNG-PM impair phagocytosis of iMGLs. a Representative images for green fluorescent and phase contrast channels showing phagocytosed green pHrodo Zymosan A bioparticles with vehicle, 50 µg ml⁻¹ EN590-PM and 50 µg CNG-PM treated cells at 6 h timepoint. Scale bars 50 µm. b Time curves for intensity of green fluorescent pHrodo bioparticles per cell density over 6-h live-cell imaging after 24-h exposure to 10, 50 and 100 µg ml-1 EN590-PM normalized to vehicle. c Time curves for 10, 50 and 100 µg ml⁻¹ CNG-PM treated iMGLs d bar graphs for three independent iMGL batches exposed to 10, 50 and 100 µg ml⁻¹ CNG-PM. e bar graphs for three independent iMGL batches exposed to 10, 50 and 100 µg ml⁻¹ EN590-PM. Data as mean ± SEM. Each batch is analysed separately using One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001; n = 3–6 wells. Cell lines MBE, MAD6, TOB064