Fig. 6

EN590- reduced and CNG-PM increases intracellular ROS production in iMGLs. a Representative green fluorescent and phase contrast images showing green signal in iMGLs at 3 h timepoint after adding the Cellrox reagent for cells that were pre-treated with vehicle, 50 µg ml⁻¹ EN590-PM or CNG-PM either for 2 h or 24 h. Scale bars 400 µm. b Time curve for the count of green fluorescent Cellrox positive cell per cell density over 6-h live-cell imaging after 2-h exposure normalized to maximum value of vehicle. c Time curve after 24-h pretreatment. d Bar graphs after 2-h exposure from 4 separate iMGL batches comparing vehicle and CNG-PM groups. e bar graphs for 24-h pre-treatment comparing vehicle and EN590 groups. Data as mean ± SEM. Student t test, done to each batch separately. **p < 0.01, ***p < 0.001; n = 3 independent experiments each with 3–6 wells. Cell lines Bioni, MBE, MAD