Fig. 9

Cytokine proteome profiler arrays of secreted cytokines in human iMGLs. Cells were stimulated for 24 h, and supernatants were harvested, pooled from three technical replicates and used for experiments. a Raw values for spot intensity in all analyzed samples presented as a heatmap after normalization to negative control and reference spots of each plot. Turquoise highly detected cytokines; white middle; grey lowly detected cytokines. b Quantification of heatmap data for vehicle, LPS and CNG50 presented as bar graphs. n = 3 biological replicates each containing 3 pooled technical replicates. c Respective fold change heatmap for mean spot intensity of EN50 (n = 1 array) compared to vehicle represented for 24 analytes with biggest differences. Data as mean ± SEM. Non-parametric Kruskal–Wallis test followed by Dunn’s test. *p < 0.05, **< 0.01, ***p < 0.001