Particulate matter from car exhaust alters function of human iPSC-derived microglia

Table 1 Chemical constituents of PM emitted from the heavy-duty bus engine fueled with EN590 diesel and from a bus fueled with compressed natural gas (CNG) [37]

Composition	ng/mg PM		ng in 50 ug PM		ng per 96 well		fg/cell
Organic constituents	EN590	CNG	EN59	CNG	EN59	CNG	EN590	CNG
PAH compounds	820	62	41	3.10	4.10	0.31	273	21
Genotoxic PAHs	380	26	19	1.30	1.90	0.13	127	9
Inorganic ions
NO₃⁻	1090	136	54.50	68.00	5.45	6.80	363	453
Na⁺	690	239	34.50	119.5	3.45	11.9	230	797
SO₄²⁻	143	777	7.15	38.85	0.72	3.89	48	259
NH₄⁺	137	199	6.85	9.95	0.69	1.00	46	66
Cl⁻	113	459	5.65	22.95	0.57	2.30	38	153
K⁺	24	116	1.20	5.80	0.12	0.58	8	39
Elemental composition
Zn	2340	228	117.0	114.0	11.70	11.4	780	760
Fe	610	169	30.50	84.50	3.05	8.45	203	563
Cu	96	214	4.80	10.70	0.48	1.07	32	71
Mn	63	94	3.15	4.70	0.32	0.47	21	31
Cr	36	226	1.80	11.30	0.18	1.13	12	75
Co	24	313	1.20	15.65	0.12	1.57	8	104
Ni	3.6	267	0.18	13.35	0.02	1.34	1.20	89
Pb	3.1	20	0.16	1.00	0.02	0.10	1.03	6.67
V	1.8	27	0.09	1.35	0.01	0.14	0.60	9.00
Cd	0.2	1.1	0.01	0.06	0.00	0.01	0.07	0.37
Total PM burden	50 ug	50 ug	50 ug	50 ug	5 ug	5 ug	0.33 ng	0.33 ng

The amount of constituents in PM presented as ng/mg PM mass and amounts used for in vitro experiments serving as an example for the specific conditions per 50 ug of PM, per a 96 well and per an individual cell
PAH polycyclic aromatic hydrocarbon

ISSN: 1743-8977