Fig. 5

Upstream Analysis to identify novel master upstream regulators and causal networks in the transcriptome datasets of tumors induced by MWCNTs and amosite asbestos. A Highly scoring upstream regulators, ranked by P-values from right-tailed Fisher's exact test. B Predicted upstream regulators of causal networks. Heat map depicts z-scores, in which orange (positive z-score) represents activation, while blue (negative z-score) represents inhibition. No color means the pathway or function being analyzed is not involved in the dataset. C A causal network with Gadd45b as an upstream regulator with 11 intervening regulators to explain differential expression of 630 genes in the transcriptome dataset MWCNT B. Shown are 13 target genes directly regulated by Jund. Graph legends: orange (activation); blue (inhibition); solid line (direct interaction); dashed line (indirect interaction); upregulated (red), downregulated (green), gray (did not meet at least one filter). Data analysis and graph generation were done using Ingenuity Pathways Analysis (IPA)