Fig. 2

Myenteric plexus of porcine duodenum. Fluorescence microscope image showing the distribution of neurons immunoreactive to PGP 9.5—used as a pan-neuronal marker and CART, GAL, nNOS, SP, VAChT, VIP in the control group (C) (A, D, G, J, M, P), after administration of low (LD) (B, E, H, K, N, R) and high (HD) (C, F, I, L, O, S) doses of MP-PET. All photographs were made by overlapping green and red fluorescent channels (green for PGP 9.5 and red for CART, GAL, nNOS, SP, VAChT and VIP, respectively). Neurons immuno-positive to a particular substance studied are indicated with arrows