Table 3 Antibodies used in the immunofluorescence method
Antigen | Species | Dilution | Code | Supplier |
|---|---|---|---|---|
PGP 9.5 | Mouse | 1:1000 | 480012 | ThermoFisher Scientific, Waltham, MA, USA |
CART | Rabbit | 1:16,000 | H-003-61 | Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA |
Gal | Guinea pig | 1:2000 | T-5036 | Peninsula, San Carlos, CA, USA |
nNOS | Rabbit | 1:3000 | PA1-033 | ThermoFisher Scientific, Waltham, MA, USA |
SP | Rat | 1:150 | 8450-0505 | BioRad, Hercules, CA, USA |
VAChT | Rabbit | 1:2000 | H-V006 | Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA |
VIP | Rabbit | 1:2000 | ab22736 | Abcam, Cambridge, United Kingdom |
AF 488 | Donkey anti-mouse IgG (H + L) | 1:1000 | A21202 | ThermoFisher Scientific, Waltham, MA, USA |
AF 546 | Donkey anti-rabbit IgG (H + L) | 1:1000 | A10040 | ThermoFisher Scientific, Waltham, MA, USA |
AF 546 | Goat anti- guinea pig IgG (H + L) | 1:1000 | A11074 | ThermoFisher Scientific, Waltham, MA, USA |
AF 546 | Goat anti-rat IgG (H + L) | 1:1000 | A11081 | ThermoFisher Scientific, Waltham, MA, USA |